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KMID : 0358320080490030266
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Korean Journal of Urology
2008 Volume.49 No. 3 p.266 ~ p.270
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Electroejaculation in the Male Rat
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Paick Sung-Hyun
Lee Byung-Ki
Yoon Sung-Wook
Baek Min-Ki
Kim Hyeong-Gon
Song Eun-Young
Lyoo Young-Soo
Lho Yong-Soo
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Abstract
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Purpose: We examined the possibility of electroejaculation for the evaluation of fertility in the male rat.
Materials and Methods: Ten 8-week-old Sprague-Dawley male rats that underwent seminal vesiculectomy were used for semen collection by electroejaculation. With a transrectal probe(60Hz, 3V, 0.5A), sign wave electric stimulation was applied to an anesthetized rat. Ejaculated semen was collected by a pipette and the volume and sperm density of the ejaculate were analyzed. Two weeks later, a second electroejaculation was performed under the same conditions with the same rats.
Results: At the first attempt, all 10 rats ejaculated following electric stimulation. The mean semen volume was 8.9?l(range, 3.0-28.5?l) and the mean sperm density was 6,428/ml(range, 320-20,997/ml). At the second attempt, only 7 of 10 rats(70%) ejaculated. The mean semen volume was 5.6?l(range, 3.3-8.6?l) and the mean sperm density was 2,801/ml (range, 227-12,555/ml).
Conclusions: These results indicated that electroejaculation has the possibility of being a useful method for evaluation of fertility in the male rat. (Korean J Urol 2008;49:268-272)
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KEYWORD
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Electric stimulation, Ejaculation, Fertility, Rats
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